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A NEW LOW-COST AND PORTABLE ELISA READER BY USING A PHOTODIODE MATRIX AND ELECTROLUMINISCENT (EL) LAMPS

Beatriz Garcia, Jesús Angel Baro, Cristina de la Torre, Rocío Muñiz, Miguel Angel Pérez

Abstract

The tool of immunological diagnosis denominated ELISA (Enzyme Linked Immuno-Sorbent Assay) it is probably the most used colorimetric test and it evaluates the compatibility antigen-antibody.<br />The development of a portable device and low cost that it is allows analyzing the ELISA plate ("in situ"). It will suppose one immediately identification of the disease and therefore a faster performance to palliate this disease. In addition to the being to low cost it will be more accessible for the population, especially of the underdeveloped countries population, where they have a depressed immunological systems as a result of the bad general condition of life; and this diseases will cause them serious injuries or even the death.<br />Another reason, but not less important it is that the presence of this disease cause great lost economic, with respect to a primary production sector like it is the farming sector, that produce a great social impact.<br />This paper presents a new portable instrument for read ELISA plates, based of the colour level (this measurement will take place in photodiodes) and using low-cost components.<br />Currently, plates are measured either photometrically which is costly and no portable or manually which is unacceptably subjective and dependent on training and illumination. This work shows a technique to improve the efficiency of clinical interpretation ELISA diagnostic plates.

Keywords
ELISA, clinical diagnosis, colorimetric measurement
Download
IMEKO-WC-2009-TC13-227.pdf
DOI
-
IMEKO TC
TC13 - Measurements in Biology and Medicine

Event details

Event
XIX IMEKO World Congress
Place
Lisbon, PORTUGAL
Time
6 September 2009 - 11 September 2009
Website
http://www.imeko2009.it.pt/

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