DEVELOPMENT OF NANO PARTICLE SIZING SYSTEM USING FLUORESCENCE POLARIZATION

Terutake Hayashi, Masaki Michihata, Yasuhiro Takaya, Kok Foong Lee

Abstract

In order to measure the sizes of nanoparticles with a wide size distribution in a solvent, we developed an optical microscopy system that allows for fluorescence polarization (FP) measurement and optical observation. This system allows the evaluation of nanoparticle sizes over a wide range, because the fluorescent signal intensity is independent of changes in the nanoparticle sizes. In this paper, we describe a fundamental experiment to verify the feasibility of using this system for different sizes of nanoparticles.

Keywords

nanoparticle, fluorescence polarization, Brownian motion, diffusion coefficient, particle sizing

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DOI

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IMEKO TC

TC2 - Photonics

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