GLUCOSE UPTAKE RATE UNCERTAINTY IN A FED BATCH BIOREACTOR

G.Sassi, M.Bernocco

Abstract

Glucose uptake rate is a typical quantity for bioreactor non invasive monitoring, which is candidate as metabolic assay for tissue engineering. Volumetric glucose uptake rate enables to compare bioreactor performances. Fed Batch reactors are typical in tissue engineering applications. This work aims to the identification of the metrological bottlenecks of an uptake rate measurement in a bioreactor for human cells; it offers a first metrological characterization of the quantities for a typical bioreactor protocol. Glucose uptake rate and volumetric one are measurable at 6% and 3% uncertainty respectively. The enhancement of bioreactor management protocols and design, and volume measurement may lead under 1% uncertainty.

Keywords

uncertainty, uptake rate, bioreactor, glucose, hMSC

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IMEKO-WC-2012-TC13-O1.pdf

DOI

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IMEKO TC

TC13 - Measurements in Biology and Medicine

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