UNCERTAINTY ANALYSIS OF CELL COUNTING BY METABOLIC ASSAYS

C.Divieto, G.Sassi, M.P.Sassi

Abstract

Cell counting is an important procedure in cell biology and its uncertainty evaluation is fundamental to improve cell-based measurement comparability which is currently variable. In this study, for the first time, the uncertainty of a metabolic assay for cell counting based on fluorescence intensity measurement has been evaluated. Results, in 2D cell culture, revealed that three are the main components: repeatability and reproducibility of the measurement system and a contribution due to the sample loading step procedure. This study represents the basis of a 3D cell culture in which metabolic methodologies are the most suitable method to monitor cell growth.

Keywords

2D and 3D cell culture, scaffold, cell proliferation rate, CellTiter Blue, resazurin, uncertainty analysis

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