STUDY ON NANOPARTICLE SIZING USING FLUORESCENT POLARIZATION METHOD WITH DNA FLUORESCENT PROBE

Terutake Hayashi, Yuki Ishizaki, Masaki Michihata, Yasuhiro Takaya, Shin-Ichi Tanaka

Abstract

A fluorescent polarization method is well known for the detection of complementary base pairing of DNA in biological field. The fluorescent polarization method (FP) measures the rotational diffusion coefficient of Brownian motion of the fluorescent particle in the solution. The rotational diffusion coefficient is corresponding to inverse third power of diameter due to the Einstein Stokes Relation for nanoparticle as hard sphere. <BR />We develop a novel rotational diffusion coefficient measurement method by using a fluorescent probe with DNA spacer, which is connected to particle. We investigate the relation between the gold nanoparticle and the fluorescent probe in order to verify the feasibility of the proposed method. The rotational diffusion coefficients of gold nanoparticles, whose diameters are from 5 nm to 20 nm, are evaluated by using the developed system. In this paper we describe the method of fluorescent polarization method by using fluorescent DNA probe (fl-DNA).

Keywords

nanoparticle sizing, fluorescent DNA probe, gold nanoparticle, fluorescent polarization, rotational diffusion coefficient, dynamic light scattering

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DOI

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IMEKO TC

TC14 - Measurement of Geometrical Quantities

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