LIGHT SCATTERING FROM SINGLE AND ORIENTED RED BLOOD CELLS: SENSITIVITY ANALYSIS

Jonas Gienger, Jörg Neukammer, Hermann Gross

Abstract

We investigate light scattering from single, randomly or actively orientated red blood cells (RBCs) employing a dedicated flow cytometer. The measured bimodal distributions of cross sections of randomly oriented RBCs can be reproduced numerically applying the discrete dipole approximation (DDA). Our sensitivity analysis of the scattering pattern reveals a significant impact of assumed biconcave shapes and different orientations of the hydrodynamically focused RBCs.

Keywords

flow cytometry, red blood cells (RBCs), discrete dipole approximation (DDA), sensitivity analysis

Download

IMEKO-WC-2015-TC21-392.pdf

DOI

-

IMEKO TC

TC21 - Mathematical Tools for Measurements

Back to the proceedings