Identification and Measurement of Botanical Species in Animal Feed

Luca Braglia, Floriana Gavazzi, Silvia Gianì, Francesco Mastromauro, Laura Morello, Diego Breviario

Abstract

Within the framework of the EU Project Feed-code, we developed and pre-validated a DNA-based protocol for the qualitative and quantitative determination of the botanical composition of dairy cow feed. Due to the biological complexity and intrinsic variability of the animal feed matrixes, we were confronted with several problematic issues. Hereby we present our current scheme and solutions, successfully tested on feed samples and raw materials.

Keywords

feed composition, raw materials, reference mixtures, DNA calibrators, target copy number

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IMEKO-TC23-2016-017.pdf

DOI

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IMEKO TC

TC23 - Metrology in Food and Nutrition

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