Chemical analysis, monitoring of dynamic changes, metabolomics and "in cell" applications of NMR in natural products and food chemistry: Is the role of metrology underestimated?
Abstract
A critical overview of recent developments of NMR spectroscopy in natural products and food chemistry will be provided with emphasis in the following applications:<BR />(i) chemical analysis of extracts without isolation or derivatization steps [1-5],<BR />(ii) ‘in situ’ direct monitoring of dynamic changes of metabolites as a function of solvent and temperature [6],<BR />(iii) aromatic C–H activation of flavonoids in aqueous solution at neutral pH and ambient temperatures [7],<BR />(iv) rapid ‘in situ’ analysis of enzymatic reaction products and enriching the biological space of natural products, through real time biotransformation monitoring [8] and<BR />(v) in-cell NMR in decoding the apoptotic activity of flavonoids with the Bcl-2 family of proteins [9].<BR />Despite the significant advantages in methodology and the fact that NMR is a potentially primary analytical technique for qualification, much effort should be made in the future so that international compatibility can be achieved for a wide range qualification, compatibility range of chemical constituents in natural products and in food chemistry. Selected examples of certified reference materials will be provided.
Event details
- Event
- 3rd IMEKOFOODS Conference
- Technical Committee
- TC23
- imekofoods3@gmail.com
- Place
- Thessaloniki, GREECE
- Time
- 1 October 2017 - 4 October 2017