Compound-specific analysis of δ¹³C and δ²H of olive oil fatty acids

Bontempo, L., Paolini, M., Camin, F.

Abstract

An analytical method was developed to measure δ¹³C, and for the first time, δ²H of fatty acid methyl esters (FAMEs) extracted from olive oil triglycerides, after base-catalysed transesterification with methanol. FAMEs are analysed using Gas Chromatography Combustion/Pyrolysis Isotope Ratio Mass Spectrometry (GCC/Py-IRMS). Accuracy, precision and uncertainty of the whole method (from preparation to measurement) were determined as well as the impact of methylation on δ¹³C and δ²H. The obtained performance of the method is satisfying and allow the determination of reliable data.

Keywords

olive oil, fatty acids, GC-CPy-IRMS, &delta;¹³<i>C</i>, &delta;²<i>H</i>

Download

IMEKO-TC23-2017-023.pdf

DOI

-

IMEKO TC

TC23 - Metrology in Food and Nutrition

Back to the proceedings