Optimisation of assay conditions for the determination of antioxidant capacity in plant food matrices by using plant food matrices by using photochemiluminescence

Zachia M., Multescu M., Iorga E.

Abstract

In this study, we optimized the extraction conditions (solvents, time of extraction, solvent-to- solid ratio, and sample storage) in order to measure the antioxidant capacity of fruits and vegetables extracts by using photochemiluminescence PCL assay. <BR />Photochem apparatus (Analytic Jena, Germany) employing the built-in protocols for water-soluble (ACW) and lipid-soluble (ACL) bioactive compounds was used for determination. We performed experiments on kiwi, lemon, lemon peel, and broccoli.

Keywords

photochemiluminescence, PCL, superoxide radical, antioxidant activity

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IMEKO TC

TC23 - Metrology in Food and Nutrition

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