Selective voltammetric determination of ascorbic acid (vitamin C) in the presence of water soluble vitamins
Abstract
Voltammetry has been considered as an important analytical technique applied to the identification and determination of trace concentrations of many biological molecules including ascorbic acid. In this study, ascorbic acid was determined by square wave voltammetry using carbon paste as working electrode modified by a film of a manganese(II) complex compound. Various factors, such as the effect of pH and some common instrumental parameters on the response characteristics of the modified electrode were investigated. From the calibration graph, the relationship between the peak height and ascorbic acid concentration within modified working electrode was investigated. The equation of the calibration graph was found to be: <i>I</i> = 0.0550 γ<sub>ac</sub> + 0.155 with <i>R</i><sup>2</sup> = 0.9998, where I is the SWV current and γ<sub>ac</sub> is the mass concentration of ascorbic acid. The LOD and LOQ for the developed method were determined to be 1.288 µg/L and 3.903 µg/L, respectively. Several compounds, such as riboflavin and biotin, and ions, such as Fe and Cu, were tested and no one of them seemed to interfere with the analytic signal. The proposed procedure was successfully applied in the determination of ascorbic acid in Rosa canina hips.
Event details
- Event
- 3rd IMEKOFOODS Conference
- Technical Committee
- TC23
- imekofoods3@gmail.com
- Place
- Thessaloniki, GREECE
- Time
- 1 October 2017 - 4 October 2017