Comparative analysis of fatty acids composition of vegetable oils and animal fats by application of GC-MS and NMR techniques

Mihai, A. L., Negoiță, M., Ionescu, V., Adascălului, A. C., Manolache, F. A., Iorga, E.

Abstract

The purpose of this paper was to compare the composition (weight % of total identified FA) in saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids from 3 food matrices (sunflower oil, palm oil and lard) by 2 different techniques, GC-MS and NMR.<BR />FAMEs analysis from food samples was performed using a gas chromatograph coupled with a Triple quadrupole mass spectrometer. <BR />NMR spectra were recorded on a Bruker Advance 400 MHz spectrometer, operating at 9.4 Tesla corresponding to the resonance frequency of 400.13 MHz for the ¹H nucleus. <BR />The composition in fatty acids determined by these two techniques was compared and the difference between the mean values determined by GC-MS and NMR was not more than ± 15% for sunflower oil, and lard, and ± 6% for palm oil.

Keywords

fatty acids, GC-MS, NMR

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IMEKO TC

TC23 - Metrology in Food and Nutrition

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