Evaluation of the biological detoxification of table olive processing wastewaters by UV-Vis spectrophotometry and liquid chromatography

Papadaki, E., Tsimidou, M. Z., Mantzouridou, F.Th.

Abstract

The contribution of UV–Vis spectrophotometry and liquid chromatography to follow phenol degradation by Aspergillus niger B60 was studied in Spanish–style green olive processing wastewaters. The percent reduction of the characteristic absorption band at 270 nm in effluent UV spectra was strongly correlated with that of the total polar phenol content estimated by the Folin– Ciocalteu assay. RP–HPLC–DAD analysis of the extracts gave more information about degradation mechanism. UV spectrophotometry can be used to monitor routinely microbial degradation of phenolic compounds in table olive processing wastewaters.

Keywords

Spanish–style green olive wastewaters, Aspergillus niger, Phenolic compound biodegradation, UV–Vis spectrophotometry, Liquid chromatography

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IMEKO TC

TC23 - Metrology in Food and Nutrition

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