Eventual limits of the current EU official method for evaluating milk adulteration of water buffalo dairy products and potential proteomic solutions

Caira, S., Nicolai, M. A., Lilla, S., Calabrese, M. G., Pinto, G., Scaloni, A., Chianese, L., Addeo, F.

Abstract

The European reference method (ERM) identifies the fraudulent addition of bovine (B) milk in water buffalo (WB) milk/dairy products by concomitant isoelectric focusing (IEF) detection of B γ2- and γ3-CN fragments after plasminolysis. False positive results could occurr as the WB β-CN (f100- 209), also formed after plasminolysis of WB milk/dairy products comigrates with B γ2-CN. These ERM limitations were overcome by a phosphopeptides enrichment procedure followed by trypsinolysis and the elution of B β-CN(f1-25)4P and WB β-CN(f1-28)4P peptides identified as suitable specie/specific markers with a detection limit of 0.8% v/v.

Keywords

gamma-2- and gamma-3-casein, peptide markers, milk, cheese, proteomics

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IMEKO TC

TC23 - Metrology in Food and Nutrition

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