Development on in -situ flow measurement technology of high performance liquid chromatography

Hai-Bo Zhao, Han Wu

Abstract

The infusion system (referred to pump) was the heart of High performance liquid chromatography (HPLC), which provided the power of HPLC. The critical parameter of the pump were the accuracy and stability of flow, which were directly related to the consistency and effectiveness of HPLC results. At present, the technical standards of HPLC are mainly JJG705-2014 and EDQM-OMCL, which were that Verification Regulation of Liquid Chromatograph and Quality Control Laboratory of European Medical Administration for flow of HPLC respective. In this study, a new portable HPLC flowmeter (PM-210plus) for HPLC was used. It adopted the volumetric principle, including the fully closed volumetric parts and the special constant force in situ detection structure, which realized in-situ measurement of HPLC flow and data traceability. The flowmeter was calibrated by Dynamic mass method water flow standard device of National Institute of metrology, China (NIM ).Parameters of flow in-situ measurement were designed, including value error, stability, and temperature influence test. After the optimization of conditions, the flow range could be covered from 0.01 mL/min to 5.0 mL/min in situ conditions, the maximum allowable error was within ± 1 %, the stability was within 0.5 %, and each measurement was completed in 3 min. It fully met the requirements of JJG705 field flow measurement, and greatly improved the field measurement efficiency.

Keywords

Flowmeter; HPLC; Calibration; In-situ measurement

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IMEKO-TC9-2019-134.pdf

DOI

10.21014/tc9-2022.134

IMEKO TC

TC9 - Flow Measurement

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