Two-photon calcium imaging experiments to investigate the properties of neuronal microcircuits: the example of cerebellar network activity

Marialuisa Tognolina, Egidio D'Angelo

Abstract

We have developed a custom-made two-photon microscope integrating a spatial light modulator that can modulate the phase of a laser beam and divide it into multiple diffraction-limited beamlets configurable in real time. We used this system to study cerebellar circuit activity and long-term synaptic plasticity expression by simultaneously acquiring stimulus-induced calcium signals from neurons located in different cerebellar layers.

Download

IMEKO-TC2-2025-018.pdf

DOI

10.21014/tc2-2025.018

IMEKO TC

TC2 - Photonics

Back to the proceedings