INFLUENCE OF OSMOTIC PRESSURE ON LIGHT SCATTERING BY RED BLOOD CELLS |
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| R. Szczepanowski, T. Guszkowski, J. Mroczka |
- Abstract:
- The paper presents results of the simulation of the light scattering phenomenon by red blood cells (RBC) under reduction of the osmotic pressure in ektacytometry. The modified anomalous diffraction theory was used to describe single light scattering by RBC for small scattering angles and allowed approximation of changing shape and size of RBC. Approximation of RBC by ellipsoidal and spheroidal particles were considered. The changes in the optical properties of the blood-sodium chloride suspension induced by the osmolality reduction have been registered in a form of the intensity patterns. Shape parameters deformability (DI) and ellipticity (EI) from the simulated intensity patterns were used for deformability evaluation. The model well approximates the behaviour of RBC in hypotonic media in ektacytometry, although the deformability curves of RBC (DI) from constant observation points deviate from the real RBC ellipticity. It is caused by the increase of the RBC volume during osmolality changes. The results showed that cellular sphericity is more significant on RBC deformability than the RBC hemoglobin optical properties.
- Keywords:
- light scattering, red blood cell, osmolality
- Download:
- IMEKO-WC-2000-TC13-P345.pdf
- DOI:
- -
- Event details
- Event name:
- XVI IMEKO World Congress
- Title:
Measurement - Supports Science - Improves Technology - Protects Environment ... and Provides Employment - Now and in the Future
- Place:
- Vienna, AUSTRIA
- Time:
- 25 September 2000 - 28 September 2000