ARTIFICIAL SYNAPSIS – THE DETECTOR OF PESTICIDE TOXICITY |
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| Jiri Haze, Michal Skocdopole, Jan Krejci, Radimir Vrba, Lukas Fujcik, Dagmar Krejcova, Zuzana Grosmanova |
- Abstract:
- Fast detection of organophosphorous pesticide toxicity in field conditions has not been fully solved yet. The new tool for field measurement of organophosporous pesticides toxicity is described. The detection is based on the inhibition of acetylcholinesterase. The enzyme acetylcholinesterase is immobilized on a miniature electrochemical detector, which is made by Thick Film Technology (TFT). The dimensions of TFT electrochemical sensor are 7,35 x 25,4 mm. The detector is placed in a narrow gap in which the analyzed sample and acetylthiocholine flow. This arrangement simulates the removal of acetylcholin from synaptic gap. It creates the simple model of synapsis - the Artificial Synapsis (AS).
The AS detects the integral sample toxicity. It enables organophosporous pesticides traces to be detected in washout from leaves as well as their direct measurement in rivers, ponds, vaste waters and drinking water sources. The preconcentration of the sample is possible. The detection limit varies in wide range depending on the toxicity of pesticide.
Structure and function of AS is described. Limit of detection (LOD) was found for pesticide Syntostigmin 10-10 mol. - Keywords:
- artificial synapse, microfluidics, organophosphorous pesticides
- Download:
- PWC-2003-TC19-017.pdf
- DOI:
- -
- Event details
- Event name:
- XVII IMEKO World Congress
- Title:
Metrology in the 3rd Millennium
- Place:
- Dubrovnik, CROATIA
- Time:
- 22 June 2003 - 28 June 2003