Determination of Calcium and Phosphor in Bones Using Microwave Digestion and ICP-MS Comparison of Manual and Automated Methods using ICP-MS |
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| Heidi Fleischer, Ellen Vorberg, Kerstin Thurow, Mareike Warkentin, Detlef Behrend |
- Abstract:
- The determination of the elemental composition of environmental samples provides information for biological monitoring in environmental and occupational health. Concentrations of selected elements contained in human bones may show the current state of health, diseases, intoxications or food pattern. Various studies show age independent concentrations of Calcium (Ca) and Phosphor (P) in human bones, but a correlation between osteoporosis and a decreased Ca concentration is reported. The combination of inductively coupled plasma and mass spectrometry (ICP-MS) enables fast multi-elemental measurements with high accuracy and relative low material consumption. In this study, three methods for the determination of P and Ca in bone materials using ICP-MS are presented and compared. This includes two manual methods and one method for automated sample preparation and subsequent analysis in an automated system, which enables an accurate and fast determination of P and Ca in bone materials. All methods were validated and tested using commercially available animal bones. For the methods presented, the repeat accuracy was determined with 39-46 pig bone samples. A concentration range of P with 93.5-100.5 g/kg and of Ca 191.2-203.2 g/kg with was determined with coefficients of variation (CV) in the range of 1.56-3.17 %. The measurement precision showed CV values of 1.07-2.08 %. A fully automated system for sample preparation, sample transportation, measurements using ICP-MS and subsequent data processing was realized and validated.
- Keywords:
- laboratory automation, automated sample preparation, ICP-MS, microwave digestion, bone material, human health, environmental monitoring
- Download:
- IMEKO-TC19-2014-020.pdf
- DOI:
- -
- Event details
- IMEKO TC:
- TC19
- Event name:
- Methods and Advances in Measurement
- Title:
5th Symposium on Environmental Instrumentation and Measurements
- Place:
- Chemnitz, GERMANY
- Time:
- 23 September 2014 - 24 September 2014