LIGHT SCATTERING FROM SINGLE AND ORIENTED RED BLOOD CELLS: SENSITIVITY ANALYSIS

Jonas Gienger, Jörg Neukammer, Hermann Gross
Abstract:
We investigate light scattering from single, randomly or actively orientated red blood cells (RBCs) employing a dedicated flow cytometer. The measured bimodal distributions of cross sections of randomly oriented RBCs can be reproduced numerically applying the discrete dipole approximation (DDA). Our sensitivity analysis of the scattering pattern reveals a significant impact of assumed biconcave shapes and different orientations of the hydrodynamically focused RBCs.
Keywords:
flow cytometry, red blood cells (RBCs), discrete dipole approximation (DDA), sensitivity analysis
Download:
IMEKO-WC-2015-TC21-392.pdf
DOI:
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Event details
Event name:
XXI IMEKO World Congress
Title:

Measurement in Research and Industry

Place:
Prague, CZECH REPUBLIC
Time:
30 August 2015 - 04 September 2015