Identification of Protein Markers for the Occurrence of Defrosted Material in Milk Through a MALDI-TOF-MS Profiling Approach |
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| Anna Maria Salzano, Simona Arena, Andrea Scaloni |
- Abstract:
- A progressive diffusion of Mozzarella cheese products realized with frozen curd or frozen milk have been observed in the last years. In order to reveal adulterations in raw milk used for cheese manufacturing, we have developed a robust and rapid MALDI-TOF-MS profiling procedure of fresh and frozen buffalo milks. We finally identified 28 polypeptide markers of milk freezing storage. Abundance changes of polipeptide species following freezing treatment were confirmed by an independent approach. We observed the progressive formation in frozen buffalo milk of GLYCAM1-derived phosphopeptides.A progressive diffusion of Mozzarella cheese products realized with frozen curd or frozen milk have been observed in the last years. In order to reveal adulterations in raw milk used for cheese manufacturing, we have developed a robust and rapid MALDI-TOF-MS profiling procedure of fresh and frozen buffalo milks. We finally identified 28 polypeptide markers of milk freezing storage. Abundance changes of polipeptide species following freezing treatment were confirmed by an independent approach. We observed the progressive formation in frozen buffalo milk of GLYCAM1-derived phosphopeptides.
- Keywords:
- milk adulteration, defrosted milk, MALDI-TOF profiling
- Download:
- IMEKO-TC23-2016-067.pdf
- DOI:
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